Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Benefits of Glutathione Glutathione 500mg Suppository Antioxidant Support Bayview Pharmacy Drug Induced Nutrient Depletion Pavilion Compounding Pharmacy Glutathione the power antioxidant Did you also know that our liver produces this naturally and only a small amount can be absorbed from the foods we eat? Book Valeo Health's IV Glutathione deficiency and heart failure: a systematic review of human and animal evidence ScienceDirect